The Pan-American Highway (French: (Auto)route panaméricaine/transaméricaine; Portuguese: Rodovia/Autoestrada Pan-americana; Spanish: Autopista/Carretera/Ruta Panamericana) is a network of roads stretching across the Americas and measuring about 30,000 kilometres (19,000 mi) in total length. Except for a break of approximately 106 km (66 mi) across the border between northwest Colombia and southeast Panama called the Darién Gap, the roads link almost all of the Pacific coastal countries of the Americas in a connected highway system. According to Guinness World Records, the Pan-American Highway is the world's longest "motorable road". It is only possible to cross by land between South America and Central America—the last town in Colombia to the first outpost in Panama—by a difficult and dangerous hike of at least four days through the Darién Gap, one of the rainiest areas of the planet.

The Pan-American Highway passes through many diverse climates and ecological types—ranging from dense jungles to arid deserts and barren tundra. Some areas are fully passable only during the dry season. The Pan-American Highway system is physically mostly complete and extends in de facto terms from Prudhoe Bay, Alaska, in North America, to the southernmost reaches of South America. Several southern highway termini are claimed, including the cities of Puerto Montt and Quellón in Chile, and Ushuaia in Argentina.

West and north of the Darién Gap, this roadway is also known as the Inter-American Highway through Central America and Mexico. There it splits into several spurs leading to the Mexico–United States border.